An analysis of the most effective disinfectant agent available to common households against Escherichia coli and Staphylococcus aureus
Md Danish Karlin, Md Danish B Mass., Tan Zhi En
School of Science and Technology, Singapore
School of Science and Technology, Singapore
The effectiveness of 9 disinfectants used in hospitals, day-care centers, and food service kitchens in killing E. coli and S. aureus colonies, hand picked by our group, was determined through the usage of the Kirby-Bauer method. E. coli and S. aureus, one of the most common bacteria found in nature, are chosen in our research.
Methods
We grew E. coli and S. aureus colonies first, to make sure that those bacteria are still living. We then chose the bacteria colonies that are healthy, and spread them onto another petri dish. We then let diffusion disks absorb the disinfectants we were testing, and put them onto the petri disks spread with the bacteria. When the bacteria grows, there would be a clearance of lawn around the diffusion disks, due to the lysis of the bacteria. The most effective disinfectant is the one that has the largest radius of clearance of lawn.
We grew E. coli and S. aureus colonies first, to make sure that those bacteria are still living. We then chose the bacteria colonies that are healthy, and spread them onto another petri dish. We then let diffusion disks absorb the disinfectants we were testing, and put them onto the petri disks spread with the bacteria. When the bacteria grows, there would be a clearance of lawn around the diffusion disks, due to the lysis of the bacteria. The most effective disinfectant is the one that has the largest radius of clearance of lawn.
Results
Based on our results, we observed that S. aureus is weaker and more vulnerable to lysis caused by the disinfectant compared to E. coli. Based on the data collected and calculated, Sodium Hypochlorite is the most effective in killing E. coli, while Hydrogen Peroxide is the most effective in eliminating S. aureus.
Conclusions
The effectiveness of all nine disinfectant agents have been assessed and tested. Based on the findings of the research, we have concluded that Sodium Hypochlorite is the most effective disinfectant agent in lysing Escherichia coli and Hydrogen Peroxide is the most effective disinfectant agent in lysing Staphylococcus aureus. The findings are very conclusive as the error bars of our results do not coincide, therefore showing the most obvious and effective disinfectants in lysing E. coli or S. aureus.
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